Involvement of cortical microtubules in plastic extension regulated by gibberellin in Lemna minor root.

We aimed to analyze the rheological characteristics during elongation of the root segments in Lemna minor. The elastic component of segment elongation (EC) increased for the first 6 h, and then almost stopped. However, the plastic component of the segment elongation (PC) began to rapidly increase from 6 h onwards. Uniconazole-P, a gibberellin biosynthesis inhibitor, inhibited the total elongation of root segments (TE), and this inhibition was mainly caused by suppression of the rapid increase in the PC after 6 h. Concomitant with this inhibition, the cortical microtubule (CMT) array within root epidermal cells became disorganized in the presence of uniconazole-P from 6 h onwards. Adding GA3 abolished the inhibition of TE by uniconazole-P treatment, and this recovery was caused not by the increase in the EC but by an increase in the PC. Furthermore, the CMT arrays also recovered their characteristic organization in the presence of GA3. These findings suggest that endogenous gibberellin accelerates TE by activating the PC via control of CMT arrays. This conclusion is also supported by rheological analysis where propyzamide was used to disrupt microtubules. We suggest that endogenous gibberellin controls the PC via its influence over the transverse arrangement of CMTs.